Cultivating Spirulina in Waste Saltwater: A Sustainable Solution for High-Protein Biomass Production from Solar Still Brine Discharge

Document Type : Research Article

Authors

1 Department of Chemical Engineering, University of sistan and Baluchestan, Zahedan, Iran.

2 Department of Chemical Engineering, Faculty of Engineering, University of Sistan and Baluchestan, Zahedan, Iran

Abstract

This study explores the cultivation of Spirulina using desalination brine waste as a growth medium, offering a sustainable method to produce high-protein biomass. Spirulina, a filamentous blue-green alga, is rich in protein and essential nutrients, making it an ideal alternative to traditional protein sources. In this research, desalination brine waste was used, and its salt concentration was optimized to create a conducive environment for Spirulina cultivation. The algae were grown under controlled light and temperature conditions, yielding 2.5 grams of dried biomass per 2 liters with a protein content of approximately 60%, along with significant amounts of carbohydrates and essential minerals like potassium, calcium, and sodium. Structural analysis confirmed the healthy growth of Spirulina filaments, and elemental analysis indicated a rich nutritional profile. These results suggest that Spirulina cultivation using desalination waste is not only feasible but also presents a highly nutritious, economically viable, and sustainable alternative protein source, particularly for athletes and health-conscious consumers. Moreover, this method provides an innovative approach to recycling desalination waste, contributing to more sustainable aquaculture and food production systems.

Keywords


[1] Bădescu, V., 1985. The influence of certain astronomical and constructive parameters on the concentration of solar radiation with plane heliostats fields. Revue de physique appliquée, 20(10), 711-733.
[2] Dorian, M.E., Nelson, D.H., 1980. Solar tracking device. U.S. Patent 4,223,214.
[3] Yilmaz, S., Ozcalik, H.R., Dogmus, O., Dincer, F., Akgol, O., Karaaslan, M., 2015. Design of two axes sun tracking controller with analytically solar radiation calculations. Renewable and Sustainable Energy Reviews, 43, 997-1005. http://dx.doi.org/10.1016/j.rser.2014.11.050
[4] Nsengiyumva, W., Chen, S.G., Hu, L., Chen, X., 2018. Recent advancements and challenges in Solar Tracking Systems (STS): A review. Renewable and Sustainable Energy Reviews, 81, 250-279. http://dx.doi.org/10.1016/j.rser.2017.06.085
[5] Eldin, S.S., Abd-Elhady, M.S., Kandil, H.A., 2016. Feasibility of solar tracking systems for PV panels in hot and cold regions. Renewable Energy, 85, 228-233. http://dx.doi.org/10.1016/j.renene.2015.06.052
[6] Kvasznicza, Z., Elmer, G., 2006. Optimizing solar tracking systems for solar cells. In Proceedings of the 4th Serbian–Hungarian Joint Symposium on Intelligent Systems, September, Subotica, Serbia, 77-84.
[7] Pelizer, L.H., Danesi, E.D.G., de O Rangel, C., Sassano, C.E., Carvalho, J.C.M., Sato, S., Moraes, I.O., 2003. Influence of inoculum age and concentration in Spirulina platensis Journal of Food Engineering, 56(4), 371-375. http://dx.doi.org/10.1016/S0260-8774(02)00268-4
[8] Kawata, Y., Yano, S.I., Kojima, H., Toyomizu, M., 2004. Transformation of Spirulina platensis strain C1 (Arthrospira PCC9438) with Tn5 transposase–transposon DNA–cation liposome complex. Marine Biotechnology, 6, 355-363. http://dx.doi.org/10.1007/s10126-004-3056-7
[9] Volkmann, H., Imianovsky, U., Oliveira, J.L., Sant'Anna, E.S., 2008. Cultivation of Arthrospira (Spirulina) platensis in desalinator wastewater and salinated synthetic medium: Protein content and amino-acid profile. Brazilian Journal of Microbiology, 39, 98-101. http://dx.doi.org/10.1590/S1517-83822008000100022
[10] Vonshak, A., ed., 1997. Spirulina platensis arthrospira: Physiology, Cell-Biology and Biotechnology. CRC Press.
[11] Venkataraman, L.V., Sindhu Kanya, T.C., 1981. Insect contamination in the mass outdoor cultures of blue green alga, Spirulina platensis. In Proceedings of the Indian Academy of Sciences, 90(6), 665-672.
[12] Thangaraj, R., Mahendran, S., Nizhanthini, C., Dhanasekaran, D., Thajuddin, N., 2023. Small/Large-Scale Production, Cost Benefit Analysis, and Marketing of Spirulina Single Cell Protein. In Food Microbiology Based Entrepreneurship: Making Money from Microbes, Springer Nature Singapore, 115-132.
[13] Rodrigues, R.D.P., de Castro, F.C., de Santiago-Aguiar, R.S., Rocha, M.V.P., 2018. Ultrasound-assisted extraction of phycobiliproteins from Spirulina (Arthrospira) platensis using protic ionic liquids as solvent. Algal Research, 31, 454-462. http://dx.doi.org/10.1016/j.algal.2018.02.016
[14] Su, C.H., Liu, C.S., Yang, P.C., Syu, K.S., Chiuh, C.C., 2014. Solid–liquid extraction of phycocyanin from Spirulina platensis: Kinetic modeling of influential factors. Separation and Purification Technology, 123, 64-68. http://dx.doi.org/10.1016/j.seppur.2013.12.019
[15] Wang, F., Yu, X., Cui, Y., Xu, L., Huo, S., Ding, Z., Hu, Q., Xie, W., Xiao, H., Zhang, D., 2023. Efficient extraction of phycobiliproteins from dry biomass of Spirulina platensis using sodium chloride as extraction enhancer. Food Chemistry, 406, 135005. http://dx.doi.org/10.1016/j.foodchem.2023.135005
[16] Bermejo, P., Piñero, E., Villar, Á.M., 2008. Iron-chelating ability and antioxidant properties of phycocyanin isolated from a protean extract of Spirulina platensis. Food Chemistry, 110(2), 436-445. http://dx.doi.org/10.1016/j.foodchem.2008.02.065
[17] Matos, A.P., da Silva, T., Sant’Anna, E.S., 2021. The feasibility of using inland desalination concentrate (DC) as an alternative substrate for Spirulina platensis mass cultivation. Waste and Biomass Valorization, 12, 3193-3203. http://dx.doi.org/10.1007/s12649-020-01291-7
[18] Samimi, M., Moghadam, H., 2024. Modified evacuated tube collector basin solar still for optimal desalination of reverse osmosis concentrate. Energy, 289, 129983. http://dx.doi.org/10.1016/j.energy.2023.129983
[19] Samimi, M., Moghadam, H., 2024. Investigation of structural parameters for inclined weir-type solar stills. Renewable and Sustainable Energy Reviews, 190, 113969. http://dx.doi.org/10.1016/j.rser.2023.113969
[20] Abrofarakh, M., Moghadam, H., Abdulrahim, H.K., 2024. Investigation of direct contact membrane distillation (DCMD) performance using CFD and Machine Learning approaches. Chemosphere, 141969. http://dx.doi.org/10.1016/j.chemosphere.2024.141969
[21] Tabrizi, F.F., Dashtban, M., Moghaddam, H., Razzaghi, K., 2010. Effect of water flow rate on internal heat and mass transfer and daily productivity of a weir-type cascade solar still. Desalination, 260(1-3), 239-247. http://dx.doi.org/10.1016/j.desal.2010.03.037